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IL-10 antibody

Cat.#: 111654

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Special Price 239.0 USD

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Product Information

  • Product Name
    IL-10 antibody
  • Documents
  • Description
    IL-10 Rabbit Polyclonal antibody. Positive WB detected in Recombinant protein, Transfected HEK-293 cells. Positive IHC detected in human pancreas cancer tissue.
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CSIF antibody; IL 10 antibody; IL10 antibody; IL-10 antibody; IL10A antibody; interleukin 10 antibody; TGIF antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of IL-10 recombinant protein (Accession Number: NM_000572). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:50-1:500

  • Validations

    Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:111654(IL10 Antibody) at dilution of 1:1000

    Recombinant protein were subjected to SDS PAGE followed by western blot with Catalog No:111654(IL10 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111654(IL10 Antibody) at dilution of 1:1000

    Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:111654(IL10 Antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:111654(IL10 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:111654(IL10 Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:111654(IL10 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using Catalog No:111654(IL10 Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

  • Background
    Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
  • References
    • Xu Q, Fu R, Yin G, Liu X, Liu Y, Xiang M. Microarray-based gene expression profiling reveals genes and pathways involved in the oncogenic function of REG3A on pancreatic cancer cells. Gene. 578(2):263-73. 2016.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"