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Product Name
IkB alpha antibody
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Description
IkB alpha Rabbit Polyclonal antibody. Positive IHC detected in human kidney tissue, human liver tissue, human prostate cancer tissue, human stomach tissue. Positive IF detected in HeLa cells. Positive WB detected in HL-60 cells, HeLa cells, Jurkat cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 39 kDa
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Tested applications
ELISA, WB, IP, IF, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
I kappa B alpha antibody; IkappaBalpha antibody; IkB alpha antibody; IKBA antibody; MAD 3 antibody; MAD3 antibody; NF kappa B inhibitor alpha antibody; NFKBI antibody; NFKBIA antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of IkB alpha recombinant protein (Accession Number: NM_020529). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:10-1:100
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Validations
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:111641(NFKBIA antibody) at dilution of 1:500
IP Result of anti-NFKBIA (IP:Catalog No:111641, 5ug; Detection:Catalog No:111641 1:500) with HeLa cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111641(NFKBIA Antibody) at dilution of 1:200 (under 10x lens).
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:111641(NFKBIA Antibody) at dilution of 1:200 (under 40x lens).
Immunofluorescent analysis of HeLa cells using Catalog No:111641(NFKBIA Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA, synonyms: IKBA, MAD-3, NFKBI). NFKB1 or NFKB2 is bound to REL, RELA , or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime.
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References
- Wang F, Yang JL, Yu KK. Activation of the NF-κB pathway as a mechanism of alcohol enhanced progression and metastasis of human hepatocellular carcinoma. Molecular cancer. 14:10. 2015.
- Tu Q, Xiong Y, Fan L. Peroxiredoxin 6 attenuates ischemia‑ and hypoxia‑induced liver damage of brain‑dead donors. Molecular medicine reports. 13(1):753-61. 2016.
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