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Product Name
Anti-IKB alpha Rabbit antibody
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Description
IKB alpha Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
I kappa B alpha;I kappa B alpha;I(Kappa)B(alpha);I(Kappa)B(alpha);I-kappa-B-alpha; IkappaBalpha;IkB-alpha;IKBA;IKBA;IKBA_HUMAN;IKBalpha;MAD 3;MAD 3;MAD3;Major histocompatibility complex enhancer binding protein MAD3;Major histocompatibility complex enhanc antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human IKB alpha
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000-1/10000
IHC: 1/20
ICC/IF: 1/20
FC: 1/20
IP: 1/20
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Validations
Western blot detection of IKB alpha in 3T3,Hela cell lysates using IKB alpha Rabbit pAb(1:1000 diluted).Predicted band size:36KDa.Observed band size:39KDa.
Western blot detection of IKB alpha in Raw264.7 cell lysates using IKB alpha Rabbit pAb(1:1000 diluted).Predicted band size:36KDa.Observed band size:39KDa.
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Background
Swiss-Prot Acc.P25963.Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"