Human PDGFRB/CD140b (His Tag) recombinant protein

Human PDGFRB/CD140b (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.

Platelet-derived growth factor receptor beta

PDGFR-1; PDGFRB; AI528809; JTK12; PDGFR; IBGC4; IMF1; CD140B; PDGFR1

Q59F04

PDGFRB; PDGFR; PDGFR1

Human Cells

A DNA sequence encoding the human PDGFR? (NP_002600.1) extracellular domain (Met 1-Lys 531) was expressed, with a C-terminal polyhistidine tag.

Human

The secreted recombinant human PDGFR? comprises 510 amino acids and predicts a molecular mass of 57.6 kDa. It migrates as an approximately 95-100 kDa band in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized PDGFRβ-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB (Cat:502517), The EC₅₀ of Cynomolgus PDGFB (Cat:502517) is 3-8 ng/ml.