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Product Name
Anti-PDGFRB/CD140b antibody
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Description
Rabbit polyclonal to PDGFRB/CD140b
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Tested applications
ELISA, IHC-P
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Species reactivity
Cynomolgus PDGFRB / CD140b
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Alternative names
AI528809 antibody; CD140b antibody; CD140B antibody; JTK12 antibody; Pdgfr antibody; PDGFR antibody; PDGFR1 antibody; Pdgfrb antibody; PDGFRB antibody; IMF1 antibody; IBGC4 antibody; JTK12 antibody; PDGFR antibody; CD140B antibody; PDGFR1 antibody; PDGFR-1 antibody; Pdgfr antibody; CD140b antibody; PDGFR-1 antibody; AI528809 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus PDGFRB / CD140b (F7BTH1; Met1-Phe530). PDGFRB / CD140b specific IgG was purified by Cynomolgus PDGFRB / CD140b affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/ml
This antibody can be used at 0.1-0.2 μg/ml with the appropriate secondary reagents to detect Cynomolgus PDGFRB / CD140b.
IHC-P: 0.1-2 μg/mL
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Validations
PDGFRB / CD140b Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of cynomolgus PDGFRB in cynomolgus brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.
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References
- Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
- Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
- Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
- Suzuki S, et al. (2010) Clinicopathological significance of platelet-derived growth factor (PDGF)-B and vascular endothelial growth factor-A expression, PDGF receptor- phosphorylation, and microvessel density in gastric cancer. BMC cancer. 10: 659.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"