Human Junctional Adhesion Molecule A (Fc Tag) recombinant protein

Human Junctional Adhesion Molecule A (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

F11 receptor

JCAM1; AA638916; JAM1; JAMA; 9130004G24; PAM-1; JCAM; JAM; ESTM33; CD321; LY106; JAM-1; F11R; KAT; JAM-A

Q9Y624

F11R; hCG_20857

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ala 242) of human JAM-A (NP_058642.1) precursor was expressed with the fused Fc region of human IgG1 at the C-terminus.

Human

The mature recombinant human JAM-A/Fc chimera is a disulfide-linked homodimeric protein. The reduced monomer comprises 453 amino acids and has a calculated molecular mass of 50 kDa. As a result of glycosylation, the rh JAM-A/Fc monomer migrates as an approximately 61 kDa protein in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 8 x 104 cells/well are added to JAM-A-Fc coated plates (2.5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37℃.