Human Junctional Adhesion Molecule A (His Tag) recombinant protein

Human Junctional Adhesion Molecule A (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Junctional adhesion molecule-A (JAM-A), also known as F11 receptor (F11R) or Cluster of Differentiation 321 (CD321), is a transmembrane protein expressed at tight junctions of epithelial and endothelial cells, as well as on circulating leukocytes. JAM-A protein serves as a serotype-independent receptor for mammalian orthoreoviruses (reoviruses). It is also a ligand for the integrin LFA1, involves in leukocyte transmigration. As a cell adhesion molecule of the immunoglobulin superfamily, JAM-A protein involves in platelet adhesion, secretion and aggregation, and plays a crucial role in inflammatory thrombosis and atherosclerosis. In addition, it may be a potential therapeutic target for breast cancer.

F11 receptor

JAM-A; KAT; F11R; JAM-1; LY106; CD321; ESTM33; JAM; JCAM; PAM-1; 9130004G24; JAMA; JAM1; AA638916; JCAM1

Q9Y624

F11R; hCG_20857

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Ala 242) of human JAM-A (NP_058642.1) precursor was expressed with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human JAM-A comprises 227 amino acids and has a calculated molecular mass of 25 kDa. As a result of glycosylation, it migrates with the molecular mass of approximately 28-32 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 8 x 104 cells/well are added to JAM-A-Fc coated plates (2.5μg/mL, 100 μL/well)in the presence of 20 ng/mL PMA, approximately 30-40% will adhere after 30 minutes at 37℃.