Human Ephrin-A3/EFNA3 (Fc Tag) recombinant protein

Human Ephrin-A3/EFNA3 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.

Ephrin-A3

EFL-2; EFNA3; MGC129301; EPL3; LERK3; LERK-3; AW494418; EPHRIN-A3; EFL2; EHK1-L; EPLG3; EPHRIN A3; MGC129300

P52797

EFNA3; EFL2; EPLG3; LERK3

Human Cells

A DNA sequence encoding the human EphrinA3 (NP_004943.1) (Met 1-Ser 213) with the C-terminal propeptide removed was fused with the Fc region of human IgG1 at the C-terminus.

Human

The recombinant human EphrinA3/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 429 amino acids and has a predicted molecular mass of 48 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhEphrinA3/Fc monomer is approximately 60-65 kDa due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse EphA6 at 1 μg/ml (100 μl/well) can bind human EphrinA3 / Fc Chimera. The EC₅₀ of human EphrinA3 is 299.2 ng/mL.