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Product Name
Anti-Ephrin-A3/EFNA3 antibody
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Description
Rabbit polyclonal to Ephrin-A3/EFNA3
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse Ephrin-A3 / EFNA3
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Alternative names
Epl3 antibody; EFL-2 antibody; Ehk1-L antibody; LERK-3 antibody; AW494418 antibody; EFL2 antibody; EPLG3 antibody; LERK3 antibody; Ehk1-L antibody; AW494418 antibody; EFL2 antibody; EFL-2 antibody; EFNA3 antibody; Efna3 antibody; Ehk1-L antibody; Ehk1-L antibody; Ephrin A3 antibody; Ephrin-A3 antibody; Epl3 antibody; EPLG3 antibody; LERK3 antibody; LERK-3 antibody; MGC129300 antibody; MGC129301 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Ephrin-A3 / EFNA3 (rh Ephrin-A3 / EFNA3; NP_034238.1; Met1-Ser205). Ephrin-A3 / EFNA3 specific IgG was purified by Mouse Ephrin-A3 / EFNA3 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse Ephrin-A3 / EFNA3. The detection limit for Mouse Ephrin-A3 / EFNA3 is <0.039 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
Ephrin-A3 / EFNA3 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse EFNA3 in mouse brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Ephrin-A3 also known as EPH-related receptor tyrosine kinase ligand 3 or EFNA3, is a member of the ephrin family. The Eph family receptor interacting proteins (ephrins) are a family of proteins that serve as the ligands of the Eph receptor, which compose the largest known subfamily of receptor protein-tyrosine kinases (RTKs). Ephrin-A3 and their Eph family of receptor tyrosine kinases are expressed by cells of the SVZ. Ephrin subclasses are further distinguished by their mode of attachment to the plasma membrane: Ephrin-A3 ligands bind EphA receptors and are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) linkage, whereas ephrin-B ligands bind EphB receptors and are anchored via a transmembrane domain. Ephrin-A3 expressed on astrocytes activates EphA4 on the post-synaptic neuron and restricts the growth of dendritic spines through multiple pathways.
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References
- Klein R. (2009) Bidirectional modulation of synaptic functions by Eph/ephrin signaling. Nat Neurosci. 12(1): 15-20.
- Lai KO, et al. (2009) Synapse development and plasticity: roles of ephrin/Eph receptor signaling. Curr Opin Neurobiol. 19(3): 275-83.
- Prevost N, et al. (2002) Interactions between Eph kinases and ephrins provide a mechanism to support platelet aggregation once cell-to-cell contact has occurred. Proc Natl Acad Sci U S A. 99(14): 9219-24.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"