Human CTLA-4/CD152 (His Tag) recombinant protein

Human CTLA-4/CD152 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).

Cytotoxic T-lymphocyte protein 4

CD152; CTLA-4; GRD4; CELIAC3; IDDM12; GSE; CH29-120A16.2; ALPS5; ICOS; CD; CTLA4; LY-56

P16410

CTLA4; CD152

Human Cells

A DNA sequence encoding the human CTLA4 (NP_005205.2) extracellular domain (Met 1-Phe 162) was fused with the a polyhistidine tag at the C-terminus.

Human

The recombinant human CTLA4 consists of 137 amino acids and has a predicted molecular mass of 15 kDa.

> 90 % as determined by SDS-PAGE

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human CTLA4 (cat: 503821) at 10 μg/mL (100 μl/well) can bind human B7-1/Fc (cat: 503507), The EC₅₀ of human B7-1/Fc (cat: 503507 is 14 ng/mL.
3. Immobilized human CTLA4 (cat: 503821) at 10 μg/mL (100 μl/well) can bind human B7-2/Fch (cat: 504521), The EC₅₀ of human B7-2/Fch (cat: 504521) is 53 ng/mL.