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Product Name
Anti-CTLA-4/CD152 antibody
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Description
Rabbit polyclonal to CTLA-4/CD152
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Tested applications
ELISA, WB
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Species reactivity
Mouse CTLA-4 / CD152
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Alternative names
ICOS antibody; CD antibody; CD152 antibody; Cd152 antibody; CELIAC3 antibody; CH29-120A16.2 antibody; Ctla4 antibody; CTLA4 antibody; CTLA-4 antibody; Ctla-4 antibody; GRD4 antibody; GSE antibody; IDDM12 antibody; Ly-56 antibody; CD antibody; GSE antibody; GRD4 antibody; ALPS5 antibody; CD152 antibody; CTLA-4 antibody; IDDM12 antibody; CELIAC3 antibody; Cd152 antibody; Ly-56 antibody; Ctla-4 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse CTLA-4 / CD152 (rM CTLA-4 / CD152; NP_033973.2; Met 1-Phe 162). Total IgG was purified by Protein A affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 20-40 μg/ml
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse CTLA4. The detection limit for Mouse CTLA4 is approximately 0.0049 ng/well.
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Validations
CTLA-4 / CD152 Antibody, Rabbit PAb, Western blot
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Background
Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2). CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg).
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References
- Slavik JM, et al. (1999) CD28/CTLA-4 and CD80/CD86 families: signaling and function. Immunol Res. 19(1): 1-24.
- Holmberg D, et al. (2005) CTLA-4 (CD152) and its involvement in autoimmune disease. Autoimmunity. 38(3): 225-33.
- Chin LT, et al. (2008) Immune intervention with monoclonal antibodies targeting CD152 (CTLA-4) for autoimmune and malignant diseases. Chang Gung Med J. 31(1): 1-15.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"