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Human CD3D & CD3E recombinant protein

Cat.#: 504825

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Product Information

  • Product Name
    Human CD3D & CD3E recombinant protein
  • Documents
  • Description
    The CD3 complex mediates signal transduction.
  • Protein name
    T-cell surface glycoprotein CD3 delta chain
  • Protein short names
    T3D
  • Uniprot ID
    P04234
  • Gene Name
    CD3D; T3D
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
  • Protein Species
    Human
  • Molecular weight
    The recombinant heterodimer of human CD3D&CD3E comprises 272 (126+146) amino acids and has a calculated molecular mass of 31.2 (14.4+16.8)KDa. As a result of glycosylation, the apparent molecular mass of human CD3D&CD3E heterodimer is approximately 20, 22 and 26 KDa in SDS-PAGE under reducing conditions.
  • Purity
    (43.8+13.1+41.9) % as determined by SDS-PAGE
  • Activity
    Measured by its binding ability in a functional ELISA.
    Immobilized human CD3D & CD3E Heterodimer Protein (Cat:CT038-H2508H) at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 7.0-18.0 ng/ml.
  • Validations

    Human CD3D & CD3E Heterodimer Protein SDS-PAGE

    Human CD3D & CD3E Heterodimer Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"