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Human CD3D & CD3E recombinant protein

Cat.#: 504646

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Product Information

  • Product Name
    Human CD3D & CD3E recombinant protein
  • Documents
  • Description
    The CD3 complex mediates signal transduction.
  • Protein name
    T-cell surface glycoprotein CD3 delta chain
  • Protein short names
    T3D
  • Uniprot ID
    P04234
  • Gene Name
    CD3D; T3D
  • Source/Expression Host
    Human Cells
  • Expression Plasmid/cDNA
    A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
  • Protein Species
    Human
  • Molecular weight
    The recombinant heterodimer of human CD3D/CD3E comprises 670 (333+354) amino acids and has a calculated molecular mass of 77.5 (37.5+40? kDa. As a result of glycosylation, the apparent molecular mass of human CD3D/CD3E heterodimer is approximately 45-50 kDa in SDS-PAGE under reducing conditions.
  • Purity
    > 90 % as determined by SDS-PAGE
  • Activity
    Measured by its binding ability in a functional ELISA.
    Immobilized human CD3D & CD3E Heterodimer Protein (Cat:CT026-H0323H) at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml.
  • Validations

    Human CD3D & CD3E Heterodimer Protein SDS-PAGE

    Human CD3D & CD3E Heterodimer Protein SDS-PAGE

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"