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Product Name
Mouse CD3D & CD3E recombinant protein
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Description
The CD3 complex mediates signal transduction.
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Protein name
T-cell surface glycoprotein CD3 delta chain
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Uniprot ID
P04235
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Gene Name
Cd3d; T3d
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
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Protein Species
Mouse
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Molecular weight
The recombinant heterodimer of mouse CD3D/CD3E comprises 254 (126+128) amino acids and has a calculated molecular mass of 29.2 (14.3+14.9) KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 20 and 22 KDa in SDS-PAGE under reducing conditions.
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Purity
(42.3+45.3) % as determined by SDS-PAGE
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Validations
Mouse CD3D & CD3E Heterodimer Protein SDS-PAGE
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