-
Product Name
HMGA1 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to HMGA1
-
Tested applications
WB, IHC
-
Species reactivity
Mouse, Rat
-
Alternative names
HMGA1 antibody; HMG-R antibody; HMGA1A antibody; HMGIY antibody; high mobility group AT-hook 1 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:1000 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - HMGA1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using HMGA1 antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - HMGA1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using HMGA1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - HMGA1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse kidney using HMGA1 antibody at dilution of 1:100 (40x lens).
-
Background
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"