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Product Name
Anti-HMGA1 Rabbit antibody
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Description
HMGA1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human
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Alternative names
HMG-R; HMGIY; HMGA1A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human HMGA1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/200
ICC/IF: 1/100
FC: 1/100
IP: 1/20
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Validations
Immunocytochemistry of HMGA1(green) in Hela cells using HMGA1 Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of HMGA1 in Hela,C6,MCF7 cell lysates using HMGA1 Rabbit pAb(1:1000 diluted).Predicted band size:12KDa.Observed band size:18KDa.
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Background
Swiss-Prot Acc.P17096.HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.RegionsFeature keyPosition(s)Description
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"