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Product Name
HMGA1 Polyclonal Antibody
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Description
Polyclonal antibody to HMGA1
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Tested applications
WB, IHC, IF
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Species reactivity
Human
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Alternative names
HMGA1 antibody; HMG-R antibody; HMGA1A antibody; HMGIY antibody; high mobility group AT-hook 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HMGA1 (NP_665908.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - HMGA1 Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using HMGA1 antibody at 1:3000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit ._Exposure time: 90s.
Immunohistochemistry - HMGA1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using HMGA1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - HMGA1 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using HMGA1 antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Background
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double-stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"