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Product Name
FKBP52 antibody
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Description
FKBP52 Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human cervical cancer tissue, human colon cancer tissue. Positive FC detected in HeLa cells. Positive WB detected in HeLa cells, K-562 cells, MCF7 cells. Observed molecular weight by Western-blot: 52 kDa
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Tested applications
ELISA, WB, IF, FC, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
51 kDa FK506 binding protein antibody; 52 kDa FK506 binding protein antibody; 52 kDa FKBP antibody; 59 kDa immunophilin antibody; FK506 binding protein 4 antibody; FK506 binding protein 4 antibody; 59kDa antibody; FKBP 4 antibody; FKBP 52 antibody; FKBP4 antibody; FKBP51 antibody; FKBP52 antibody; FKBP59 antibody; HBI antibody; HSP binding immunophilin antibody; Hsp56 antibody; Immunophilin FKBP52 antibody; p52 antibody; p59 antibody; PPIase antibody; PPIase FKBP4 antibody; Rotamase antibody
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Isotype
Mouse IgG
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Preparation
This antibody was obtained by immunization of FKBP52 recombinant protein (Accession Number: NM_002014). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107217(FKBP52 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:107217(FKBP52 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:107217(FKBP52 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0
Immunofluorescent analysis of HeLa cells using Catalog No:107217(FKBP52 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG
1X10^6 HeLa cells were stained with 0.2ug FKBP52 antibody (Catalog No:107217, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000.
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Background
FKBP52 (FK506 binding protein 4) is a member of the immunophilin protein family that comprises intracellular protein effectors of immunosuppressive drugs, and it is known as a steroid receptor-associated protein. As a novel regulator of microtubule dynamics, FKBP52 is associated with the motor protein dynein and with the cytoskeleton during mitosis. FKBP52 is highly expressed in CNS regions susceptible to Alzheimer's, and plays a role in modulating toxicity of Abeta peptides. The protein can associate with the Tau function, and may help to decipher and modulate the events involved in Tau-induced neurodegeneration. In addition, FKBP52 is likely to play a role in growth and development of the male genitalia.
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