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Product Name
Cynomolgus CD32a / Fc gamma RIIA / FCGR2A (His & AVI Tag) recombinant protein
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Protein name
Fc gamma receptor IIa
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Protein short names
FCGR21; RP11-5K23.6
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Uniprot ID
Q8SPW4
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32a (AAL92096.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
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Protein Species
Cynomolgus
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Molecular weight
The recombinant cynomolgus CD32a consists of 206 amino acids and has a calculated molecular mass of 22.9 kDa. The apparent molecular mass of the recombinant protein is approximately 33-35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Labeling ratio of biotin to protein: 1.1
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Validations
Cynomolgus CD32a / FCGR2A Protein (His & AVI Tag), Biotinylated SDS-PAGE
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