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Product Name
Human CD32a / Fc gamma RIIA / FCGR2A (167 His, His & AVI Tag) recombinant protein
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Protein name
Low affinity immunoglobulin gamma Fc region receptor II-a
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Protein short names
FCGR21; RP11-5K23.6
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Uniprot ID
P12318
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Gene Name
FCGR2A; CD32; FCG2; FCGR2A1; IGFR2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. It is identical to FCGR2A131H/R in the reference.
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Protein Species
Human
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Molecular weight
The recombinant human CD32aconsists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 33 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
1. Measured by its binding ability in a functional ELISA. Immobilized human IgG2 at 10 μg/ml (100 μl/well) can bind biotinylated human CD32 with a linear ranger of 0.16-0.8 μg/ml.
2. Using the Octet RED System, the affinity constant (Kd) of CD32a-AVI-His + BirA bound to human IgG was 12nM.
3. Labeling ratio of biotin to protein: 0.1-0.6. -
Validations
Human CD32a / FCGR2A Protein (167 His, His & AVI Tag), Biotinylated SDS-PAGE
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