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Product Name
Human CD32a / Fc gamma RIIA / FCGR2A (167 Arg, His Tag) recombinant protein
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Protein name
Low affinity immunoglobulin gamma Fc region receptor II-a
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Protein short names
FCGR21; RP11-5K23.6
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Uniprot ID
P12318
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Gene Name
FCGR2A; CD32; FCG2; FCGR2A1; IGFR2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) precursor was expressed, with a C-terminal polyhistidine tag. It is identical to FCGR2A131H/R in the reference.
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Protein Species
Human
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Molecular weight
The recombinant human CD32a consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD32a at 10 μg/ml (100 μl/well) can bind human IgG2 with a linear range of 12.5-200 ng/ml.
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Validations
Human CD32a / FCGR2A Protein (167 Arg, His Tag) SDS-PAGE
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