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Product Name
Human CD32a / Fc gamma RIIA / FCGR2A (His & AVI Tag) recombinant protein
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Protein name
Low affinity immunoglobulin gamma Fc region receptor II-a
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Protein short names
RP11-5K23.6; FCGR21
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Uniprot ID
P12318
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Gene Name
FCGR2A; CD32; FCG2; FCGR2A1; IGFR2
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain (Met 1-Met 210) of human CD32a (P12318-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. It is identical to FCGR2A131H/R in the reference.
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Protein Species
Human
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Molecular weight
The recombinant human CD32a consists of 213 amino acids after removal of the signal peptide and predicts a molecular mass of 23.6 KDa. It migrates as an approximately 32 KDa band due to glycosylation by SDS-PAGE under reducing conditions.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by its binding ability in a functional ELISA.
Immobilized human CD32a-AVI (167 Arg/His)at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.13-0.29 μg/ml. -
Validations
Human CD32a / FCGR2A Protein (167 His) (His & AVI Tag) SDS-PAGE
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