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Product Name
Human EphB3/HEK2/Eph Receptor B3 (aa 585-998, His & GST Tag) recombinant protein
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Description
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.
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Protein name
Ephrin type-B receptor 3
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Protein short names
MDK5; AW456895; CEK10; EPHB3; ETK2; TYRO6; HEK2; SEK4
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Uniprot ID
P54753
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Gene Name
EPHB3; ETK2; HEK2; TYRO6
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Source/Expression Host
Baculovirus-Insect Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the human EPHB3 (P54753) (Gln585-Val998) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human EPHB3 /GST chimera consists of 651 amino acids and has a calculated molecular mass of 74.7 kDa. The recombinant protein migrates as an approximately 64 kDa band in SDS-PAGE under reducing conditions.
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Purity
> 90 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 90 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
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Validations
Human EphB3 / HEK2 Protein (aa 585-998, His & GST Tag) SDS-PAGE
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