Rat EphB3/HEK2/Eph Receptor B3 (His Tag) recombinant protein

Rat EphB3/HEK2/Eph Receptor B3 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Receptor protein-tyrosine kinase

EPHB3; MDK5; ETK2; TYRO6; AW456895; HEK2; CEK10; SEK4

D3ZH39

Ephb3; Ephb3_predicted; rCG_36502

Human Cells

A DNA sequence encoding the rat Ephb3 (NP_001099338.1) (Met1-Thr537) was expressed with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat Ephb3 consists 519 amino acids and predicts a molecular mass of 57 kDa.

> 95 % as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA.
Immobilized rat EPHB3-His at 10 μg/ml (100 μl /well) can bind rat EFNB1-Fc(Cat:502474), The EC₅₀ of rat EFNB1-Fc(Cat:502474) is 2-6ng/ml.