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Product Name
Anti-EphB3/HEK2/Eph Receptor B3 antibody
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Description
Rabbit polyclonal to EphB3/HEK2/Eph Receptor B3
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Tested applications
ELISA, IHC-P
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Species reactivity
Human EPHB3 / HEK2
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Alternative names
Etk2 antibody; HEK2 antibody; MDK5 antibody; Sek4 antibody; Cek10 antibody; Tyro6 antibody; AW456895 antibody; ETK2 antibody; HEK2 antibody; TYRO6 antibody; AW456895 antibody; Cek10 antibody; Etk2 antibody; HEK2 antibody; HEK2 antibody; MDK5 antibody; TYRO6 antibody; Ephb3 antibody; EphB3 antibody; ETK2 antibody; Sek4 antibody; Tyro6 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human EPHB3 / HEK2 (rh EPHB3 / HEK2; P54753; Gln585-Val998). EPHB3 / HEK2 specific IgG was purified by Human EPHB3 / HEK2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human EPHB3 / HEK2. The detection limit for Human EPHB3 / HEK2 is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
EphB3 / HEK2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EPHB3 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
EphB3 / HEK2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EPHB3 in human cirrhosis with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
EphB3 / HEK2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EPHB3 in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
EphB3 / HEK2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EPHB3 in human colon with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
EphB3 / HEK2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human EPHB3 in human gastric cancer with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induce various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.
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References
- Bergemann AD, et al. (1998) Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 16(4): 471-80.
- Hock B, et al. (1998) PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor. Proc Natl Acad Sci U S A. 95(17): 9779-84.
- Liu X, et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J Neurosci. 26(12): 3087-101.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"