Mouse EphB3/HEK2/Eph Receptor B3 (His Tag) recombinant protein

Mouse EphB3/HEK2/Eph Receptor B3 (His Tag) recombinant protein

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Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Ephrin type-B receptor 3

MDK5; CEK10; AW456895; ETK2; TYRO6; EPHB3; SEK4; HEK2

P54754

Ephb3; Etk2; Mdk5; Sek4

Human Cells

A DNA sequence encoding the mouse EPHB3 (NP_034273.1) extracellular domain (Met 1-Thr 537) was expressed, fused with a polyhistidine tag at the C-terminus.

Mouse

The secreted recombinant mouse EPHB3 consists of 519 amino acids and has a predicted molecular mass of 57 kDa. It migrates as an approximately 63 kDa band in SDS-PAGE under reducing conditions.

> 94 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 μg/ml (100 μl/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/ml.