Cynomolgus PD-L1/B7-H1/CD274 (His Tag) recombinant protein

Cynomolgus PD-L1/B7-H1/CD274 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Programmed cell death 1 ligand 1

B7-H; MGC142296; MGC142294; CD274 A530045L16RIK; PDCD1LG1; PDL1; PDCD1L1; B7H1; PD-L1; CD274

F6VEW6

CD274; EGK_07725

Human Cells

A DNA sequence encoding the cynomolgus/rhesus CD274 (F6VEW6) (Met1-Thr239) was expressed with a polyhistidine tag at the C-terminus. Cynomolgus and Rhesus CD274 sequences are identical.

Cynomolgus

The recombinant cynomolgus/rhesus CD274 comprises 232 amino acids and has a calculated molecular mass of 26.7 KDa. The apparent molecular mass of it is approximately 37 KDa in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized Cynomolgus CD274-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDCD1-Fc (Cat：90311-C02H), The EC₅₀ of Cynomolgus PDCD1-Fc (Cat：90311-C02H) is 1.0-2.3μg/ml.