Human CXADR / CAR (His Tag) recombinant protein

Human CXADR / CAR (His Tag) recombinant protein

• DataSheet
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Component of the epithelial apical junction complex that may function as an homophilic cell adhesion molecule and is essential for tight junction integrity. Also involved in transepithelial migration of leukocytes through adhesive interactions with AMICA1/JAML a transmembrane protein of the plasma membrane of leukocytes. The interaction between both receptors also mediates the activation of gamma-delta T-cells, a subpopulation of T-cells residing in epithelia and involved in tissue homeostasis and repair. Upon epithelial CXADR-binding, AMICA1 induces downstream cell signaling events in gamma-delta T-cells through PI3-kinase and MAP kinases. It results in proliferation and production of cytokines and growth factors by T-cells that in turn stimulate epithelial tissues repair.

Coxsackievirus and adenovirus receptor

CXADR; HCAR; MCAR; AW553441; CAR; 2610206D03RIK; MCVADR; AU016810; CAR4/6

P78310

CXADR; CAR

Human Cells

A DNA sequence encoding the human CXADR (NP_001329.1) extracellular domain (Met 1-Gly 237) was fused with a polyhistidine tag at the C-terminus.

Human

The secreted recombinant human CXADR consists of 229 amino acids and has a predicted molecular mass of 25.6 kDa. As a result of glycosylation, the apparent molecular mass of rh CXADR is approximately 35 kDa in SDS-PAGE under reducing conditions.

> 92 % as determined by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of mouse neutrophils. When 5 x 10E4 cells/well are added to CXADR coated plates (4 μg/ml and 100 μl/well), approximately 20%-40% will adhere specifically after 60 minutes at 37 ℃.