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Product Name
Anti-CXADR / CAR antibody
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Description
Rabbit polyclonal to CXADR / CAR
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse CXADR
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Alternative names
CAR antibody; 2610206D03Rik antibody; AU016810 antibody; AW553441 antibody; CAR antibody; CXADR antibody; Cxadr antibody; HCAR antibody; MCAR antibody; MCVADR antibody; CAR antibody; HCAR antibody; CAR4/6 antibody; CAR antibody; MCAR antibody; MCVADR antibody; AU016810 antibody; AW553441 antibody; 2610206D03Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse CXADR (rM CXADR; NP_001020363.1; Met 1-Gly 237). CXADR specific IgG was purified by Mouse CXADR affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CXADR. The detection limit for Mouse CXADR is approximately 0.00975 ng/well.
IHC-P: 0.1-2 μg/mL
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Validations
CXADR Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CXADR in mouse liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
CXADR Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse CXADR in mouse stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.
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References
- Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352–3356.
- van Raaij , M.J. et al., 2001, Structure. 8 (11): 1147–1155.
- Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392–25398.
- Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219–226.
- Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127–131.
- Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111.
- Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.
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