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Product Name
Anti-CXADR / CAR antibody
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Description
Rabbit monoclonal to CXADR / CAR
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Tested applications
FCM, IF, ICC/IF
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Species reactivity
Human CXADR / CAR
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Alternative names
CAR antibody; 2610206D03Rik antibody; AU016810 antibody; AW553441 antibody; CAR antibody; CXADR antibody; Cxadr antibody; HCAR antibody; MCAR antibody; MCVADR antibody; CAR antibody; HCAR antibody; CAR4/6 antibody; CAR antibody; MCAR antibody; MCVADR antibody; AU016810 antibody; AW553441 antibody; 2610206D03Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CXADR / CAR (rh CXADR / CAR; NP_001329.1; Met1-Gly237).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
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Validations
CXADR / CAR Antibody, Rabbit MAb, Flow cytometric analysis
Flow cytometric analysis of Human CXADR expression on HT-29 cells. Cells were stained with purified anti-Human CXADR, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells.
CXADR / CAR Antibody, Rabbit MAb
Immunofluorescence staining of Human CXADR in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CXADR monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cells membrane.
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Background
CXADR (coxsackie virus and adenovirus receptor), also known as CAR, is a type I transmembrane glycoprotein belonging to the CTX family of the Ig superfamily, and is essential for normal cardiac development in the mouse. Proposed as a homophilic cell adhesion molecule, CXADR is a component of the epithelial apical junction complex that is essential for the tight junction integrity, and probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN). Mature mouse CXADR structrually comprises a 218 aa extracellular domain (ECD) with a V-type (D1) and a C2-type (D2) Ig-like domain, a 21 aa transmembrane segment and a 107 aa intracellular domain, among which,D1 is thought to be responsible for homodimer formation in trans within tight junctions. The ECD of mouse CXADR shares 97%, 90% sequence identity with the corresponding regions of rat, human CXADR.
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References
- Tomko, R.P. et al., 1997, Proc. Natl. Acad. Sci. U.S.A. 94 (7): 3352–3356.
- van Raaij , M.J. et al., 2001, Structure. 8 (11): 1147–1155.
- Cohen, C.J. et al., 2001, J. Biol. Chem. 276 (27): 25392–25398.
- Carson, S.D. et al., 2002, Rev. Med. Virol. 11 (4): 219–226.
- Selinka, H.C. et al., 2004, Med. Microbiol. Immunol. 193 (2-3): 127–131.
- Philipson, L. et al., 2004, Curr. Top. Microbiol. Immunol. 273:87-111.
- Raschperger, E. et al., 2006, Exp. Cell Res. 312: 1566-1580.
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