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Product Name
Cofilin antibody
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Description
Cofilin Mouse Monoclonal antibody. Positive IF detected in Hela cells. Positive IHC detected in human testis tissue. Positive WB detected in HeLa cells, human heart tissue, Neuro-2a cells, rat brain tissue. Observed molecular weight by Western-blot: 18 kDa
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Tested applications
ELISA, IHC, IF, WB
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
18 kDa phosphoprotein antibody; CFL antibody; CFL1 antibody; Cofilin antibody; Cofilin 1 antibody; cofilin 1 (non muscle) antibody; Cofilin antibody; non muscle isoform antibody; p18 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of Cofilin recombinant protein (Accession Number: NM_005507). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107168(Cofilin Antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human testis using Catalog No:107168(CFL1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:107168(CFL1 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using CFL1 antibody Catalog No: at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"