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Product Name
Anti-Phospho-Cofilin (Ser3) Rabbit antibody
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Description
Phospho-Cofilin (Ser3) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: Peptide sequence around phosphorylation site of serine 3 (M-A-S(p)-G-V) derived from Human cofilin.
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Clonality
Polyclonal
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Formulation
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB:1/500-1/1000ICC:1/100-1/200IHC:1/50-1/100
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Validations
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cofilin (Phospho-Ser3) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Western blot detection of cofilin(Phospho-Ser3) in Hela cells untreated or treated with H2O2 using cofilin(Phospho-Ser3) Rabbit pAb (dilution 1:500, upper) or u03b2-Actin Mouse mAb (200068-8F10, lower).Predicted band size:19kDa.Observed band size:19kDa.
Western blot analysis of extracts from COLO205 cells using cofilin (Phospho-Ser3) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).
Immunofluorescence staining of methanol-fixed Hela cells using cofilin (Phospho-Ser3) Antibody .
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Background
Swiss-Prot Acc.P23528.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"