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Product Name
CNPY2,MSAP antibody
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Description
CNPY2,MSAP Rabbit Polyclonal antibody. Positive FC detected in HepG2 cells. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in mouse pancreas tissue, Huh7 cells, human liver tissue. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IHC, IP, IF, FC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
canopy 2 homolog (zebrafish) antibody; CNPY2 antibody; HP10390 antibody; MSAP antibody; Protein canopy homolog 2 antibody; TMEM4 antibody; Transmembrane protein 4 antibody; ZSIG9 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CNPY2,MSAP recombinant protein (Accession Number: NM_014255). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
WB result of anti-CNPY2 (Catalog No:109356, 1:2000) with 40ug Huh7 cells by Dr.Dan Lindholm.
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with Catalog No:109356(CNPY2,MSAP antibody) at dilution of 1:300
IP Result of anti-CNPY2,MSAP (IP:Catalog No:109356, 3ug; Detection:Catalog No:109356 1:500) with HepG2 cells lysate 600ug.
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109356(CNPY2,MSAP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:109356(CNPY2,MSAP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:109356(CNPY2,MSAP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 HepG2 cells were stained with 0.2ug CNPY2,MSAP antibody (Catalog No:109356, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.
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Background
CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2
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References
- Do HT, Tselykh TV, Mäkelä J. Fibroblast growth factor-21 (FGF21) regulates low-density lipoprotein receptor (LDLR) levels in cells via the E3-ubiquitin ligase Mylip/Idol and the Canopy2 (Cnpy2)/Mylip-interacting saposin-like protein (Msap). The Journal of biological chemistry. 287(16):12602-11. 2012.
- Do HT, Bruelle C, Tselykh T, Jalonen P, Korhonen L, Lindholm D. Reciprocal regulation of very low density lipoprotein receptors (VLDLRs) in neurons by brain-derived neurotrophic factor (BDNF) and Reelin: involvement of the E3 ligase Mylip/Idol. The Journal of biological chemistry. 288(41):29613-20. 2013.
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