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Product Name
Anti-CNPY2 antibody
- Documents
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Description
Rabbit polyclonal to CNPY2
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human CNPY2
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Alternative names
MSAP antibody; TMEM4 antibody; ZSIG9 antibody; HP10390 antibody; Tmem4 antibody; Zsig9 antibody; AW229003 antibody; D10Bwg1546e antibody; 5330432A10Rik antibody; 5330432A10Rik antibody; AW229003 antibody; canopy 2 homolog (zebrafish) antibody; CNPY2 antibody; Cnpy2 antibody; D10Bwg1546e antibody; HP10390 antibody; MIR-interacting saposin-like protein antibody; MIR-interacting saposin-like protein antibody; MSAP antibody; protein canopy homolog 2 antibody; protein canopy homolog 2 antibody; putative secreted protein ZSIG9 antibody; putative secreted protein Zsig9 antibody; Tmem4 antibody; TMEM4 antibody; transmembrane protein 4 antibody; transmembrane protein 4 antibody; UNQ1943/PRO4426 antibody; Zsig9 antibody; ZSIG9 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CNPY2 (rh CNPY2; Q9Y2B0-1; Met1-Ser178). CNPY2 specific IgG was purified by Human CNPY2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CNPY2. The detection limit for Human CNPY2 is < 0.039 ng/well.
IHC-P: 0.05-1 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
CNPY2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CNPY2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CNPY2 in human pancreas with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
CNPY2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CNPY2 in human placenta with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
CNPY2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
CNPY2 is a novel MIR-interacting protein that enhances neurite outgrowth and increases myosin regulatory light chain. CNPY2 enhances migration of C6 glioma cells through phosphorylation of the myosin regulatory light chain. It is expressed in different tissues, including brain. Overexpression of CNPY2 enhanced the motility of glioma cells measured in matrigel invasion chambers and using a scratch assay. Downregulation of CNPY2 by RNA interference significantly decreased glioma cell migration and phosphorylation of MRLC. Inhibition of the corresponding MRLC kinase by ML-7 did not affect migration of CNPY2-overexpressing cells.
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References
- Trynka G, et al. (2009) Coeliac disease-associated risk variants in TNFAIP3 and REL implicate altered NF-kappaB signalling. Gut. 58(8):1078-83.
- Danielsen JM, et al. (2011) Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level. Mol Cell Proteomics. 10(3):M110.003590.
- Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell. 44(2):325-40.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"