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Product Name
CNPY2, MSAP antibody
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Description
CNPY2, MSAP Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HepG2 cells, human placenta tissue, rat liver tissue. Observed molecular weight by Western-blot: 21 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
canopy 2 homolog (zebrafish) antibody; CNPY2 antibody; CNPY2 antibody; MSAP antibody; HP10390 antibody; MSAP antibody; Protein canopy homolog 2 antibody; TMEM4 antibody; Transmembrane protein 4 antibody; ZSIG9 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of CNPY2, MSAP recombinant protein (Accession Number: NM_014255). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:107167(CNPY2,MSAP Antibody) at dilution of 1:1000
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using Catalog No:107167(CNPY2,MSAP Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L)
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Background
CNPY2, also named as MSAP, TMEM4 and ZSIG9, is a positive regulator of neurite outgrowth. It interacts with the ezrin-radixin-moesin (ERM)-like myosin regulatory light chain-interacting protein (MIR), and the two proteins are co-localized in cell lines and in primary neurons. CNPY2 can stabilize MRLC and thus bring about an increase in neurite outgrowth. (PMID: 12826659 ) This antibody is specific to CNPY2
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