Product Name

CKMT1A antibody

Documents

Description

CKMT1A Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive IP detected in mouse heart tissue. Positive WB detected in rat heart tissue, human colon tissue, human testis tissue, mouse heart tissue, mouse testis tissue. Observed molecular weight by Western-blot: 45 kDa

Tested applications

ELISA, IHC, IP, WB

Species reactivity

Human,Mouse,Rat; other species not tested.

Alternative names

CKMiMi antibody; CKMT antibody; CKMT1 antibody; CKMT1A antibody; FLJ50967 antibody; Mia CK antibody; U MtCK antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of CKMT1A recombinant protein (Accession Number: NM_001015001). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

Validations

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109330(CKMT1A antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:109330(CKMT1A antibody) at dilution of 1:50 (under 40x lens)

rat heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:109330(CKMT1A Antibody) at dilution of 1:1500

IP Result of anti-CKMT1A (IP:Catalog No:109330, 4ug; Detection:Catalog No:109330 1:1000) with mouse heart tissue lysate 3200ug.

Background

CKMT1A(Creatine kinase U-type, mitochondrial) is also named as CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphogens. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

References

Svensson PA, Jernås M, Sjöholm K. Gene expression in human brown adipose tissue. International journal of molecular medicine. 27(2):227-32. 2011.
Datler C, Grimm S. Reconstitution of CKMT1 expression fails to rescue cells from mitochondrial membrane potential dissipation: implications for controlling RNAi experiments. Biochimica et biophysica acta. 1833(12):2844-55. 2013.
Enooku K, Nakagawa H, Soroida Y. Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients. International journal of cancer. 135(4):871-9. 2014.
Svensson PA, Lindberg K, Hoffmann JM. Characterization of brown adipose tissue in the human perirenal depot. Obesity (Silver Spring, Md.). 22(8):1830-7. 2014.

CKMT1A antibody

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