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Product Name
Anti-CKMT1A antibody
- Documents
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Description
Rabbit polyclonal to CKMT1A
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Tested applications
ELISA, WB, IP
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Species reactivity
Human CKMT1A
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Alternative names
mitochondrial antibody; acidic-type mitochondrial creatine kinase antibody; CKMT1 antibody; creatine kinase antibody; creatine kinase U-type antibody; mia-CK antibody; mitochondrial 1 (ubiquitous) antibody; ubiquitous mitochondrial creatine kinase antibody; U-MtCK antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CKMT1A (rh CKMT1A; P12532-1; Ala40-His417). CKMT1A specific IgG was purified by Human CKMT1A affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CKMT1A. The detection limit for Human CKMT1A is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
CKMT1A Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
CKMT1A Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
CKMT1A belongs to the ATP:guanido phosphotransferase family. It contains 1 phosphagen kinase C-terminal domain and 1 phosphagen kinase N-terminal domain. CKMT1A gene is one of two genes which encode the ubiquitous mitochondrial creatine kinase (CKMT1). CKMT1 is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. CKMT1 occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric CKMT1.
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References
- Haas RC, et al. (1989) Isolation and characterization of the gene and cDNA encoding human mitochondrial creatine kinase. J Biol Chem. 264(5):2890-7.
- Stachowiak O, et al. (1998) Oligomeric state and membrane binding behaviour of creatine kinase isoenzymes: implications for cellular function and mitochondrial structure. Mol Cell Biochem. 184(1-2):141-51.
- Lipskaya TY. (2001) Mitochondrial creatine kinase: properties and function. Biochemistry Mosc. 66(10):1098-111.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"