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Product Name
CHGA Polyclonal Antibody
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Description
Polyclonal antibody to CHGA
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
CHGA antibody; CGA antibody; chromogranin-A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 230-380 of human CHGA (NP_001266.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - CHGA Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using CHGA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - CHGA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse brain using CHGA antibody at dilution of 1:150 (40x lens).
Immunohistochemistry - CHGA Polyclonal Antibody
Immunohistochemistry of paraffin-embedded mouse spleen using CHGA antibody at dilution of 1:150 (40x lens).
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Background
Pancreastatin: Strongly inhibits glucose induced insulin release from the pancreas.; Catestatin: Inhibits catecholamine release from chromaffin cells and noradrenergic neurons by acting as a non-competitive nicotinic cholinergic antagonist. Displays antibacterial activity against Gram-positive bacteria S.aureus and M.luteus, and Gram-negative bacteria E.coli and P.aeruginosa. Can induce mast cell migration, degranulation and production of cytokines and chemokines. Acts as a potent scavenger of free radicals in vitro. May play a role in the regulation of cardiac function and blood pressure.; Serpinin: Regulates granule biogenesis in endocrine cells by up-regulating the transcription of protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway. This leads to inhibition of granule protein degradation in the Golgi complex which in turn promotes granule formation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"