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Product Name
Anti-Chga antibody
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Description
Rabbit polyclonal antibody to Chga
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Tested applications
WB, IHC-P, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
Ch antibody; cgA antibody; ChrA antibody
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was peptide
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:2,000
IHC-P: 1:200
FC: 1:100-1:200
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Validations
Fig1: Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of Lovo cells with chromogranin A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
- Background
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References
- Bauer, J.W., et al. 1993. Histamine induces a gene-specific synthesis regulation of secretogranin II but not of chromogranin A and B in chromaffin cells in a calcium-dependent manner. J. Biol. Chem. 268: 1586-1589.
- Schmid, K.W., et al. 1993. Chromogranin A, secretogranin II and vasoactive intestinal peptide in phaeochromocytomas and ganglioneuromas. Histopathology 22: 527-533.
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