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Product Name
CD45 antibody
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Description
CD45 Mouse Monoclonal antibody. Positive WB detected in pig spleen tissue, Raji cells. Positive IHC detected in human tonsillitis tissue. Positive FC detected in Raji cells. Observed molecular weight by Western-blot: 220 kDa
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Tested applications
ELISA, IHC, WB, FC
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Species reactivity
Human, Pig; other species not tested.
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Alternative names
B220 antibody; CD45 antibody; CD45R antibody; GP180 antibody; L CA antibody; LCA antibody; LeukOCyte common antigen antibody; LY5 antibody; PTPRC antibody; RPTPC antibody; T200 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of CD45 recombinant protein (Accession Number: BC014239). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IHC: 1:20-1:200
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Validations
pig spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:107137(CD45 Antibody) at dilution of 1:2000
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107137(CD45 Antibody) at dilution of 1:300 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using Catalog No:107137(CD45 Antibody) at dilution of 1:300 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"