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Anti-Ptprc antibody

Cat.#: 175318

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-Ptprc antibody
  • Documents
  • Description
    Rabbit polyclonal antibody to Ptprc
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    Lca antibody; RT7 antibody; CD45 antibody; L-CA antibody; T200 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was klh conjugated synthetic peptide derived from rat cd45/b220 501-600/1273
  • Clonality
    Polyclonal
  • Formulation
    Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Storage instructions
    Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
  • Applications

    WB:1:500-2000

    FC:1μg/Test

  • Validations

    Fig1: Blank control: Raji(blue).; Primary Antibody:Rabbit Anti- CD45 antibody , Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

    Fig1: Blank control: Raji(blue).; Primary Antibody:Rabbit Anti- CD45 antibody , Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA;; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

    Fig2: Sample:; Uterus (Mouse) Lysate at 40 ug; Large intestine (Mouse) Lysate at 40 ug; Primary: Anti-CD45 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 137 kD; Observed band size: 130 kD

    Fig2: Sample:; Uterus (Mouse) Lysate at 40 ug; Large intestine (Mouse) Lysate at 40 ug; Primary: Anti-CD45 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 137 kD; Observed band size: 130 kD

    Fig3: Sample:; MCF-7 (Human) Lysate at 40 ug; Primary: Anti-CD45 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 137 kD; Observed band size: 130 kD

    Fig3: Sample:; MCF-7 (Human) Lysate at 40 ug; Primary: Anti-CD45 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 137 kD; Observed band size: 130 kD

    Fig4: Blank control: Molt4.; Primary Antibody (green line): Rabbit Anti-CD45 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig4: Blank control: Molt4.; Primary Antibody (green line): Rabbit Anti-CD45 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig5: Blank control: Jurkat.; Primary Antibody (green line): Rabbit Anti-CD45 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig5: Blank control: Jurkat.; Primary Antibody (green line): Rabbit Anti-CD45 antibody ; Dilution: 2μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG .; Secondary Antibody : Goat anti-rabbit IgG-PE; Dilution: 1μg /test.; Protocol; The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

    Fig6: Blank control: Molt4.; Primary Antibody (green line): Rabbit Anti-CD45/PTPRC/FITC Conjugated antibody (175318#-FITC); Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG-FITC .; Protocol; The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

    Fig6: Blank control: Molt4.; Primary Antibody (green line): Rabbit Anti-CD45/PTPRC/FITC Conjugated antibody (175318#-FITC); Dilution: 1μg /10^6 cells;; Isotype Control Antibody (orange line): Rabbit IgG-FITC .; Protocol; The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

  • Background
    P04157

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"