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Anti-CD45 (8C1) Mouse antibody

Cat.#: 168151

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-CD45 (8C1) Mouse antibody
  • Documents
  • Description
    CD45 (8C1) Mouse monoclonal antibody
  • Tested applications
    ICC/IF, WB, IHC-P
  • Species reactivity
    Human
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Synthetic Peptide of CD45
  • Clonality
    Monoclonal
  • Formulation
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    IF: 1/50-200

    WB: 1/2000

    IHC: 1/50-300

  • Validations

    Western blot analysis of Hela, diluted at 1:2000.

    Western blot analysis of Hela, diluted at 1:2000.

    Western blot detection of CD45 in Jurkat cell lysates using CD45 (8C1) Mouse mAb(1:1000 diluted).Predicted band size:147KDa.Observed band size:180 to 240KDa.

    Western blot detection of CD45 in Jurkat cell lysates using CD45 (8C1) Mouse mAb(1:1000 diluted).Predicted band size:147KDa.Observed band size:180 to 240KDa.

    Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

    Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1,CD45 Monoclonal Antibody(12A9) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

    Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

    Immunofluorescence analysis of human-liver-cancer tissue. 1,CD45 Monoclonal Antibody(12A9)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

  • Background
    Swiss-Prot Acc.P06800.Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN . Dephosphorylates LYN, and thereby modulates LYN activity.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"