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Product Name
CD34 antibody
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Description
CD34 Rabbit Polyclonal antibody. Positive IHC detected in human hysteromyoma tissue, human endometrial cancer tissue, human gliomas tissue, human liver cancer tissue, human liver tissue, human renal cell carcinoma tissue, human small intestine tissue. Positive WB detected in HL-60 cells. Observed molecular weight by Western-blot: 105-120 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
CD34 antibody; CD34 molecule antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD34 recombinant protein (Accession Number: NM_001025109). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Immunohistochemical of paraffin-embedded human hysteromyoma using Catalog No:109030(CD34 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human hysteromyoma using Catalog No:109030(CD34 antibody) at dilution of 1:50 (under 40x lens)
HL-60 cells were subjected to SDS PAGE followed by western blot with Catalog No:109030(CD34 antibody) at dilution of 1:500
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Background
CD34 is a surface glycophosphoprotein expressed on developmentally early lymphohematopoietic stem and progenitor cells with a molecular weight of about 105-120 kDa. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.
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References
- Li YW, Qiu SJ, Fan J. Intratumoral neutrophils: a poor prognostic factor for hepatocellular carcinoma following resection. Journal of hepatology. 54(3):497-505. 2011.
- Chen HH, Weng BQ, Cheng KJ, Liu HY, Wang SQ, Lu YY. Effect of the vascular endothelial growth factor expression level on angiopoietin-2-mediated nasopharyngeal carcinoma growth. Vascular cell. 6(1):4. 2014.
- Qi C, Wang L, Zhang M. Serum Renalase Levels Correlate with Disease Activity in Lupus Nephritis. PloS one. 10(10):e0139627. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"