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Product Name
Mouse CD34 (His Tag) recombinant protein
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Description
Cluster of Differentiation 34 (CD34) is a member of a family of single-pass transmembrane sialomucin proteins, and may function as a cell-cell adhesion factor. CD34 protein is selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. It has been widely used as a stem and progenitor cell marker, and clinical CD34+ stem cell transplantation (CD34+SCT) has been performed for tumor purging. CD34 monoclonal antibodies are widely used to identify and isolate hemopoietic progenitors and to classify acute and chronic leukemias.
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Protein name
Putative uncharacterized protein
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Protein short names
CD34; RP11-328D5.2; AU040960
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Uniprot ID
Q543Y2
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Gene Name
Cd34
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the extracellular domain of mouse CD34 (NP_001104529.1) (Met 1-Thr 287) was expressed, with a polyhistidine tag at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse CD34 consists of 264 amino acids and has a predicted molecular mass of 28.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmCD34 is approximately 50-60 kDa due to glycosylation.
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Purity
> 98 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion of the HUVEC human umbilical vein endothelial cell line. When 4 x 10E4 cells/well are added to mouse CD34 coated plates (0.8 μg/ml, 100 μl/well), approximately >30 % will adhere after one hour at 37 ℃.
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Validations
Mouse CD34 Protein (His Tag) SDS-PAGE
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