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Product Name
CD146/MCAM antibody
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Description
CD146/MCAM Rabbit Polyclonal antibody. Positive WB detected in A375 cells, HEK-293 cells, HeLa cells. Positive IP detected in HeLa cells, HepG2 cells. Positive IHC detected in human spleen tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human ovary tumor tissue, human placenta tissue, human testis tissue. Observed molecular weight by Western-blot: 113-120 kDa
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Tested applications
ELISA, IHC, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CD146 antibody; MCAM antibody; MUC18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of CD146/MCAM recombinant protein (Accession Number: NM_006500). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:109054(CD146, MCAM Antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109054(CD146, MCAM antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human spleen using Catalog No:109054(CD146, MCAM antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-CD146, MCAM (IP:Catalog No:109054, 4ug; Detection:Catalog No:109054 1:1500) with HeLa cells lysate 4000ug.
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Background
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677).
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References
- Ji Y, Shen M, Wang X. Comparative proteomic analysis of primary schwann cells and a spontaneously immortalized schwann cell line RSC 96: a comprehensive overview with a focus on cell adhesion and migration related proteins. Journal of proteome research. 11(6):3186-98. 2012.
- Wu Z, Wu Z, Li J. MCAM is a novel metastasis marker and regulates spreading, apoptosis and invasion of ovarian cancer cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine. 33(5):1619-28. 2012.
- Wang WM, Zhao ZL, Zhang WF, Zhao YF, Zhang L, Sun ZJ. Role of hypoxia-inducible factor-1α and CD146 in epidermal growth factor receptor-mediated angiogenesis in salivary gland adenoid cystic carcinoma. Molecular medicine reports. 12(3):3432-8. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"