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Product Name
Anti-CD146/MCAM antibody
- Documents
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Description
Rabbit polyclonal to CD146/MCAM
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Mouse CD146 / MCAM
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Alternative names
CD146 antibody; MUC18 antibody; CD146 antibody; CD149 antibody; Muc18 antibody; s-endo antibody; AV025631 antibody; 1-gicerin antibody; s-gicerin antibody; 1-gicerin antibody; AV025631 antibody; CD146 antibody; CD146 antibody; CD149 antibody; gicerin antibody; Gicerin antibody; Mcam antibody; MCAM antibody; Muc18 antibody; MUC18 antibody; s-endo antibody; s-gicerin antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD146 / MCAM (rM CD146 / MCAM; Q8R2Y2-1; Met1-Val563). CD146 / MCAM specific IgG was purified by Mouse CD146 / MCAM affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CD146 / MCAM. The detection limit for Mouse CD146 / MCAM is approximately 0.039 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 4-6 μg/mg of lysate
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Validations
CD146 / MCAM Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of mouse MCAM in mouse stomach with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
CD146 / MCAM Antibody, Rabbit PAb, Antigen Affinity Purified
CD146 / MCAM Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
The CD146 antigen, also known as melanoma cell adhesion molecule (MCAM) and MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. CD146 contains the characteristic immunoglobulin-like domains (V-V-C2-C2-C2), a transmembrane region and a short cytoplasmic tail. The CD146 expression is detected in endothelial cells in vascular tissue throughout the body, and plays a role in cell adhesion, as well as in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. As a Ca2+-independent cell adhesion molecule involved in heterophilic cell to cell interactions and a surface receptor, CD146 triggers tyrosine phosphorylation of FYN and PTK2 and subsequently induced signal transduction, proteolysis, or immune recognition. This protein is also expressed predominantly on metastatic lesions and advanced primary tumours, and thus has been suggested to play an important role in tumour progression and the development of metastasis in certain human carcinomas.
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References
- Mills L, et al. (2002) Fully human antibodies to MCAM/MUC18 inhibit tumor growth and metastasis of human melanoma. Cancer Res. 62(17): 5106-14.
- Taira E, et al. (2004) Characterization of Gicerin/MUC18/CD146 in the rat nervous system. J Cell Physiol. 198(3): 377-87.
- Fritzsche FR, et al. (2008) CD146 protein in prostate cancer: revisited with two different antibodies. Pathology. 40(5): 457-64.
- Bidlingmaier S, et al. (2009) Identification of MCAM/CD146 as the target antigen of a human monoclonal antibody that recognizes both epithelioid and sarcomatoid types of mesothelioma. Cancer Res. 69(4): 1570-7.
- Boneberg EM, et al. (2009) Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix metalloprotease-dependent process. Microvasc Res. 78(3): 325-31.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"