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CD146/MCAM antibody

Cat.#: 107045

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Special Price 239.0 USD

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Product Information

  • Product Name
    CD146/MCAM antibody
  • Documents
  • Description
    CD146/MCAM Mouse Monoclonal antibody. Positive FC detected in HeLa cells. Positive IHC detected in human placenta tissue. Positive WB detected in A375 cells, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 120 kDa
  • Tested applications
    ELISA, WB, IHC, FC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    CD146 antibody; MCAM antibody; MUC18 antibody
  • Isotype
    Mouse IgG1
  • Preparation
    This antibody was obtained by immunization of CD146/MCAM recombinant protein (Accession Number: NM_006500). Purification method: Protein G purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:20-1:200

  • Validations

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:107045(CD146, MCAM antibody) at dilution of 1:1000

    A375 cells were subjected to SDS PAGE followed by western blot with Catalog No:107045(CD146, MCAM antibody) at dilution of 1:1000

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:107045(CD146, MCAM antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:107045(CD146, MCAM antibody) at dilution of 1:200 (under 10x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:107045(CD146, MCAM antibody) at dilution of 1:200 (under 40x lens)

    Immunohistochemical of paraffin-embedded human placenta using Catalog No:107045(CD146, MCAM antibody) at dilution of 1:200 (under 40x lens)

    1X10^6 HeLa cells were stained with 0.2ug CD146, MCAM antibody (Catalog No:107045, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:100.

    1X10^6 HeLa cells were stained with 0.2ug CD146, MCAM antibody (Catalog No:107045, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:100.

  • Background
    CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"