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Caspase 9/p35/p10 antibody

Cat.#: 107034

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Product Information

  • Product Name
    Caspase 9/p35/p10 antibody
  • Documents
  • Description
    Caspase 9/p35/p10 Mouse Monoclonal antibody. Positive IHC detected in human pancreas tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells. Observed molecular weight by Western-blot: 46 kDa,36 kDa
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human, Mouse; other species not tested.
  • Alternative names
    APAF 3 antibody; APAF3 antibody; Apoptotic protease Mch 6 antibody; CASP 9 antibody; CASP9 antibody; Caspase 9 antibody; Caspase 9/p35/p10 antibody; CASPASE 9c antibody; Caspase9 antibody; Caspase-9 antibody; ICE LAP6 antibody; ICE like apoptotic protease 6 antibody; MCH6 antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of Caspase 9/p35/p10 recombinant protein (Accession Number: NM_001229). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107034(Caspase 9 Antibody) at dilution of 1:1000

    HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107034(Caspase 9 Antibody) at dilution of 1:1000

    IP Result of anti-Caspase 9 (IP:Catalog No:107034, 5ug; Detection:Catalog No:107034 1:500) with HeLa cells lysate 3200ug.

    IP Result of anti-Caspase 9 (IP:Catalog No:107034, 5ug; Detection:Catalog No:107034 1:500) with HeLa cells lysate 3200ug.

    Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107034(Caspase 9 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107034(Caspase 9 Antibody) at dilution of 1:50 (under 10x lens)

    Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107034(Caspase 9 Antibody) at dilution of 1:50 (under 40x lens)

    Immunohistochemistry of paraffin-embedded human pancreas tissue slide using Catalog No:107034(Caspase 9 Antibody) at dilution of 1:50 (under 40x lens)

  • Background
    Caspase 9, apoptosis-related cysteine protease (CASP9,synonyms: MCH6, APAF3, APAF-3, ICE-LAP6, CASPASE-9c)is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. Capase 9 is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade.
  • References
    • Hu L, Su C, Song X. Tetrachlorobenzoquinone triggers the cleavage of Bid and promotes the cross-talk of extrinsic and intrinsic apoptotic signalings in pheochromocytoma (PC) 12 cells. Neurotoxicology. 49:149-57. 2015.
    • Chen J, Wang C, Lan W. Gliotoxin Inhibits Proliferation and Induces Apoptosis in Colorectal Cancer Cells. Marine drugs. 13(10):6259-73. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"