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Product Name
Anti-Caspase-9 (1D1) Mouse antibody
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Description
Caspase-9 (1D1) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Alternative names
APAF 3;APAF-3;APAF3;Apoptosis related cysteine peptidase;Apoptotic protease activating factor 3;Apoptotic protease Mch 6;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;CASP 9;CASP-9;CASP9;CASP9_HUMAN;Caspase 9 apoptosis related cysteine p antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human Caspase-9 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
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Validations
Western blot detection of Caspase-9 in A549,MCF7,Jurkat,K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.
Western blot detection of Caspase-9 in CHO-K1 cell lysate(B) and CHO-K1 transfected by Caspase-9(A) cell lysate using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49/37KDa.
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Background
Swiss-Prot Acc.P55211.Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"