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Product Name
beta Arrestin 1 antibody
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Description
beta Arrestin 1 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in HepG2 cells. Positive IP detected in mouse lung tissue. Positive WB detected in mouse lung tissue, A549 cells, U-937 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ARB1 antibody; ARR1 antibody; ARRB1 antibody; Arrestin beta 1 antibody; arrestin antibody; beta 1 antibody; Beta arrestin 1 antibody; β-Arrestin-1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of beta Arrestin 1 recombinant protein (Accession Number: NM_004041). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse lung tissue were subjected to SDS PAGE followed by western blot with Catalog No:117125(ARRB1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:117125(ARRB1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:117125(ARRB1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using ARRB1 antibody Catalog No:117125 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-Arrestin (IP:Catalog No:117125, 4ug; Detection:Catalog No:117125 1:500) with mouse lung tissue lysate 4000ug.
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Background
ARRB1 represents arrestin, beta 1
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References
- Kraemer A, Barjaktarovic Z, Sarioglu H. Cell survival following radiation exposure requires miR-525-3p mediated suppression of ARRB1 and TXN1. PloS one. 8(10):e77484. 2013.
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